Este proyecto nuclea varias líneas de investigación, todas relacionadas a los mecanismos moleculares y procesos desencadenados por el estrés oxidativo tales como la apoptosis y la autofagia en diferentes condiciones fisiopatológicas. Las líneas son: 1) Diabetes mellitus tipo 1: efecto de naringina (NAR) sobre tejidos que regulan la homeostasis del calcio extracelular; 2) Mecanismos moleculares desencadenados por NAR sobre la calcificación vascular en la diabetes mellitus tipo 1; 3) Calcitriol y drogas que deplecionan glutatión como agentes antitumorales: mecanismos moleculares en cáncer de mama y colon; 4) Tratamiento combinado de metformina y NAR para la reversión de las alteraciones metabólicas sistémicas y óseas en el síndrome metabólico experimental; 5) Evaluación del rol de Quercetina en la protección de la subfertilidad masculina asociada a un modelo experimental de síndrome metabólico; 6) modelos experimentales de hipertensión arterial: alteraciones óseas e inmunológicas y efectos preventivos de Co-enzimaQ10.
Integrantes del Grupo de Trabajo:
Directora:
Dra. Gabriela Picotto: Investigadora Independiente – Profesora Adjunta.
Vicedirectora:
Dra. Gabriela Edith Díaz de Barboza: Profesora Asociada.
Investigadores:
Dra. Valeria Andrea Rodríguez: Investigadora Adjunta – Profesora Asistente.
Dra. María Angélica Rivoira: Profesora Asistente.
Dra. Nori Tolosa de Talamoni: Investigadora Principal Ad. Honorem – Profesora Emérita.
Dra. Romina Kohan: Profesora Asistente.
Dr. Alejandro Collin: Profesor Asistente, CPA.
Dra. Luciana Moine: Profesora Asistente.
Bioq. Solange Natali Guizzardi: Profesora Asistente.
Dra. María Victoria Scalerandi: Profesor Asistente.
Lic. Nicolás Luciani: Profesor Asistente.
Lic. María Agustina Rizzi: Becaria del CONICET – Profesional Adscripta.
Lic. Guadalupe Teixeira: Becaria de SECyT-UNC – Profesional Adscripta.
Lic. Constanza Quiroga: Becaria del CONICET.
Publicaciones 2012-2022:
-New perspectives in melatonin uses. A. Carpentieri, G. Díaz de Barboza, V. Areco, M. Peralta López, N. Tolosa de Talamoni. Pharmacol. Research 65:437-444. 2012. (review)
-Sodium deoxycholate inhibits duodenal calcium absoprtion through oxidative stress and apoptosis. Rivoira M., Marchionatti A., Centeno V., Peralta López M. and Tolosa de Talamoni, N. Comparative Biochemistry & Physiology [A]. 162: 397-405. 2012.
-Buthionine sulfoximine and 1,25-dihydroxyvitamin d induce apoptosis in breast cancer cells via induction of reactive of reactive oxygen species. Bohl L., Liaudat C., Picotto G., Marchionatti A., Narvaez C., Welsh J., Rodriguez V. and Tolosa de Talamoni, N. Cancer Investigation 30: 560-570. 2012.
-Molecular aspects of vitamin D anticancer activity. G. Picotto, A. Liaudat, L. Bohl and N. Tolosa de Talamoni. Cancer Investigation 30: 604-614. 2012. (review)
-Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms. Marchionatti A., Pacciaroni A. and Tolosa de Talamoni, N. Comp. Biochem. & Physiol. Part A 164: 215-220. 2013.
-Ursodeoxycholic acid and deoxycholic acid: a good and a bad bile acid for the intestinal Ca2+ absorption. Rodríguez V., Rivoira M., Marchionatti A., Pérez A. and Tolosa de Talamoni N. Arch. of Biochem.& Biophysics 540: 19-25. 2013.
–Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione. Carpentieri A., Marchionatti A., Areco V., Pérez A., Centeno V. and Tolosa de Talamoni, N. Molecular and Cellular Biochemistry 387: 197-205. 2014.
–Oxidative stress, cell cycle arrest and differentiation contribute to the antiproliferative action of BSO and calcitriol on Caco-2 cells. Liaudat A., Bohl L., Tolosa de Talamoni N., Maletto B., Pistoresi-Palencia M., Picotto G. Anticancer Drugs 25: 810-818. 2014.
-Association of celular and molecular alterations in Leydig cells with germ cell apoptosis in testes of Graomys griseoflavus x Graomys centralis male hybrids. Díaz de Barboza G., Rodríguez V., Ponce R., Theiler G., Maldonado C. and Tolosa de Talamoni, N. Acta Histochemica 116: 604- 614. 2014.
-Past, Present and Future of Melatonin´s Clinical Uses. N. Tolosa de Talamoni, V. Areco, M. Peralta López, G. Díaz de Barboza, M. Rivoira and A. Carpentieri. En: Melatonin and Melatoninergic Drugs in Clinical Practice (Ed. V. Srinivasan et al.). Springer, India. Pág. 27-42. 2014.
–Time dependent changes in the intestinal Ca2+ absorption in rats with type I Diabetes mellitus are associated with alterations in the intestinal redox state. Rivoira M., Rodríguez V., Peralta López M. y Tolosa de Talamoni, N. Biochimica et Biophysica Acta. Molecular Basis of Disease 1852:386-94. 2015.
-Molecular aspects of the intestinal calcium absorption. Díaz de Barboza G., Guizzardi S and Tolosa de Talamoni N. World Journal of Gastroenterology 21: 7142- 7154. 2015. (review)
-Dietary and pharmacological compounds altering the intestinal Ca2+ absorption in humans and animals. Areco V., Rivoira M., Rodriguez V., Marchionatti A., Carpentieri A. y Tolosa de Talamoni, N. Nutrition Research Review 28: 83-99. 2015. (review)
-Ursodeoxycholic and deoxycholic acids: differential effects on intestinal Ca2+ uptake, apoptosis and autophagy of rat intestine. Valeria A. Rodríguez, María A. Rivoira, Adriana del V. Pérez, Ana M. Marchionatti and Nori G. Tolosa de Talamoni. Arch. Biochem. and Biophys 591:28-34. 2016.
-Melatonin not only restores but also prevents the inhibition of the intestinal Ca2+ absorption caused by glutathione depleting drugs. Vanessa Areco, Valeria Rodríguez, Ana Marchionatti, Agata Carpentieri y Nori Tolosa de Talamoni. Comp. Biochem. and Physiol. [A] 197: 16-22. 2016.
-Lithocholic acid: a new emergent protector of the intestinal calcium absorption under oxidant conditions. Ana M. Marchionatti, Adriana del V. Pérez, María A. Rivoira, Valeria A. Rodríguez and Nori G. Tolosa de Talamoni. Biochem. and Cell Biol. 95: 273-279. 2017.
-Oxidative stress, antioxidants and intestinal calcium absorption. Díaz de Barboza G., Guizzardi S., Moine L. and Tolosa de Talamoni N. World J. Gastroenterol. 23: 2841-2853. 2017. (review)
-Glutamine protects intestinal calcium absorption against oxidative stress and apoptosis. Moine L., Díaz de Barboza G., Pérez A., Benedetto M. and Tolosa de Talamoni N. Comp. Biochem. and Physiol. [A] 212:64-71. 2017.
-Combined calcitriol and menadione reduces experimental murine triple negative breast tumor. Bohl L., Guizzardi S., Rodriguez V., Hinrichsen L., Rozados V., Cremonezzi D, Tolosa de Talamoni N., Picotto G. Biomedicine and Pharmacotherapy 94: 21-26. 2017.
-Naringin prevents the inhibition of intestinal Ca2+ absorption induced by a fructose rich diet. V. Rodriguez, M. Rivoira, S. Guizzardi and N. Tolosa de Talamoni. Arch. Biochem. and Biophys. 636: 1-10. 2017.
-Naringin prevents bone loss in a rat model of type 1 Diabetes mellitus. M. Rivoira, V. Rodriguez, G. Picotto, R. Battaglino and N. Tolosa de Talamoni. Arch. Biochem. Biophys. 637: 56-63. 2018.
-Molecular mechanisms triggered by bile acids on intestinal Ca2+ absorption. A. Marchionatti, M. Rivoira, V. Rodríguez, A. Pérez and N. Tolosa de Talamoni. Current Medicinal Chemistry 25: 2122-2132. 2018. (review)
-Naringin attenuates liver damage in streptozotocin-induced diabetic rats. Rodriguez V., Plavnik L. y Tolosa de Talamoni, N. Biomed Pharmacother.105:95-102. 2018.
-Glutathione depleting drugs, antioxidants and intestinal calcium absorption. Moine L., Rivoira M., Díaz de Barboza G., Pérez A. and Tolosa de Talamoni, N. World Journal of Gastroenterology. 2018.
-Protective role of melatonin on retinal ganglionar cell: In vitro an in vivo evidences. Del Valle Bessone C, Fajreldines HD, de Barboza GED, Tolosa de Talamoni NG, Allemandi DA, Carpentieri AR, Quinteros DA. Life Sci. 218:233-240. 2019.
-Glutamine protects both transcellular and paracellular pathways of chick intestinal calcium absorption under oxidant conditions, L. Moine, A. Pérez, C. Maldonado, N. Tolosa de Talamoni and G. Díaz de Barboza, Comparative Biochem. & Physiol. [A], Mol Integr Physiol.238:110553, 2019.
–Reactive oxygen species in cancer: a paradox between pro- and anti-tumour activities. Kohan R., Collin A., Guizzardi S., Tolosa de Talamoni N. and Picotto G., Cancer Chemotherapy and Pharmacology, 86: 1-13, doi: 10.1007/s00280-020-04103-2. 2020.
–Intestinal Ca2+ absorption revisited: A molecular and clinical approach. Areco V., Kohan R., Talamoni G., Tolosa de Talamoni N., Peralta López M., World Journal of Gastroenterology, 26: 3344- 3364, doi: 10.3748/wjg.v26.i24.3344. 2020.
-Combined treatment of menadione and calcitriol increases the antiproliferative effect by promoting oxidative/nitrosative stress, mitochondrial dysfunction and autophagy in breast cancer MCF-7 cells”, S. Guizzardi, G. Picotto, V. Rodriguez, J. Welsh, C. Narvaez, L. Bohl and N. Tolosa de Talamoni, Canadian Journal of Physiology and Pharmacology, Canadian Journal of Physiology and Pharmacology, 98: 548-556, doi: 10.1139/cjpp-2019-0585. 2020.
-Role of mitochondria in the differential action of sodium deoxycholate and ursodeoxycholic acid on rat duodenum», Adriana Pérez, María Angélica Rivoira, Valeria Rodríguez, Ana Marchionatti and Nori Tolosa de Talamoni, Canadian Journal of Physiology and Pharmacology, 99:270-277, doi: 10.1139/cjpp-2019-0561. 2021.
-New Perspectives in the Pharmacological Potential of Naringin in Medicine. Rivoira M., Rodriguez V., Talamoni G., Tolosa de Talamoni N., Current Med Chem., (28), 10: 1987 – 2007. doi: 10.2174/0929867327666200604171351. 2021.
-Interaction between bovine mammary epithelial cells and planktonic or biofilm Staphylococcus aureus: The bacterial lifestyle determines its internalization ability and the pathogen recognition. Bohl L., Isaac P., Breser M., Orellano M., Correa S., Tolosa de Talamoni N., Porporatto C. Microbial Pathogenesis, 152:104604. doi: 10.1016/j.micpath.2020.104604. 2021.
–Synthesis, localization and biological effects of melatonin on intact and diseased intestine. Díaz de Barboza G., Kohan R., Picotto G., Talamoni G., Collin A., Areco V., Peralta López M and Tolosa de Talamoni N. Capítulo escrito por invitación para el libro «Melatonin: Production, Functions and Benefits» (Nova Science Publishers, Inc., New York). (Ed. Francisco López Muñoz, Alejandro Romero y Javier Egea), págs. 313-338. 2021.
-Analysis of the molecular mechanisms by flavonoids with potential use for osteoporosis prevention or therapy. Valeria Rodríguez, María Rivoira, Gabriela Picotto, Gabriela Díaz de Barboza, Alejandro Collin, Nori Tolosa de Talamoni. Current Medical Chemistry. Oak Park, Estados Unidos. ISSN: 0929-8673. DOI: 10.2174/0929867328666210921143644, 2021.
-Naringin prevents bone damage in the experimental metabolic syndrome induced by a fructose rich diet. Rivoira M., Rigalli A., Corball L., Tolosa de Talamoni N., Rodríguez V. Applied Physiology, Nutrition and Metabolism. Canada. ISSN: 1715-5312. DOI: 10.1139/apnm-2021-0473l, 2021.
-Melatonin enhances anti-tumoral effects of menadione on colon cancer cells. Collin A, Kohan R, Tolosa de Talamoni N, Picotto G. Anti-Cancer Agents in Medicinal Chemistry. 22(13):2411-2418, doi: 10.2174/1871520621666211207141729. 2022.
-Analysis of the Molecular Mechanisms by Flavonoids with Potential Use for Osteoporosis Prevention or Therapy (Revisión). Rodríguez V, Rivoira M, Picotto G, Díaz de Barboza G, Collin A and Tolosa de Talamoni N. Current Medicinal Chemistry. 29(16): 2913-2936, DOI: 10.2174/0929867328666210921143644. 2022.
-Naringin prevents diabetic nephropathy in rats through blockage of oxidative stress and attenuation of the mitochondrial dysfunction» (10.1139/cjpp-2022-0449). Pérez A, Mukdsi J, Valdez L, Rukavina-Mikusic I, Díaz de Barboza G, Tolosa de Talamoni, N, Rivoira MA. Canadian Journal of Physiology and Pharmacology. 2023, En prensa.