Homeostasis del calcio extra e intracelular y vitamina D

Este proyecto nuclea varias líneas de investigación, todas relacionadas con la regulación de los niveles de calcio extra e intracelular y el rol que le cabe a la vitamina D o sus metabolitos. Esas líneas comprenden: 1) Diabetes mellitus tipo 1: efecto de naringina sobre tejidos que regulan la homeostasis del calcio extracelular, 2) Efectos regulatorios de glutamina sobre la absorción intestinal de calcio bajo condiciones de estrés oxidativo, 3) Efecto de los ácidos biliares sobre el metabolismo energético a nivel intestinal. Implicancias en la absorción del calcio, 4) Mecanismos antineoplásicos de calcitriol y drogas que deplecionan glutatión en cáncer de mama.

 

Integrantes del Grupo de Trabajo:

Directora:

Dra. Nori Tolosa de Talamoni: Investigadora Principal

Investigadores:

Dra. Valeria Andrea Rodríguez: Investigadora Adjunta – Profesora Asistente.

Dra. María Angélica Rivoira: Profesora Asistente.

Dra. Gabriela Picotto: Investigadora Independiente – Profesora Adjunta.

Dra. Adriana del Valle Pérez: Profesora Adjunta.

Dra. Gabriela Edith Díaz de Barboza: Profesora Adjunta.

Dra. Romina Kohan: Profesora Asistente.

Dr. Alejandro Collin: Profesor Asistente, CPA.

Dra. Luciana Moine: Profesora Asistente.

Bioq. Solange Natali Guizzardi: Profesora Asistente.

Med. Germán Talamoni: Profesor Asistente.

 

Publicaciones 2012-2020:

-New perspectives in melatonin uses. A. Carpentieri, G. Díaz de Barboza, V. Areco, M. Peralta López, N. Tolosa de Talamoni. Pharmacol. Research 65:437-444. 2012. (review)

-Sodium deoxycholate inhibits duodenal calcium absoprtion through oxidative stress and apoptosis. Rivoira M., Marchionatti A., Centeno V., Peralta López M. and Tolosa de Talamoni, N. Comparative Biochemistry & Physiology [A]. 162: 397-405. 2012.

-Buthionine sulfoximine and 1,25-dihydroxyvitamin d induce apoptosis in breast cancer cells via induction of reactive of reactive oxygen species. Bohl L., Liaudat C., Picotto G., Marchionatti A., Narvaez C., Welsh J., Rodriguez V. and Tolosa de Talamoni, N. Cancer Investigation 30: 560-570. 2012.

-Molecular aspects of vitamin D anticancer activity. G. Picotto, A. Liaudat, L. Bohl and N. Tolosa de Talamoni. Cancer Investigation 30: 604-614. 2012. (review)

-Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms. Marchionatti A., Pacciaroni A. and Tolosa de Talamoni, N. Comp. Biochem. & Physiol. Part A 164: 215-220. 2013.

-Ursodeoxycholic acid and deoxycholic acid: a good and a bad bile acid for the intestinal Ca2+ absorption. Rodríguez V., Rivoira M., Marchionatti A., Pérez A. and Tolosa de Talamoni N. Arch. of Biochem.& Biophysics 540: 19-25. 2013.

Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione. Carpentieri A., Marchionatti A., Areco V., Pérez A., Centeno V. and Tolosa de Talamoni, N. Molecular and Cellular Biochemistry 387: 197-205. 2014.

Oxidative stress, cell cycle arrest and differentiation contribute to the antiproliferative action of BSO and calcitriol on Caco-2 cells. Liaudat A., Bohl L., Tolosa de Talamoni N., Maletto B., Pistoresi-Palencia M., Picotto G. Anticancer Drugs 25: 810-818. 2014.

-Association of celular and molecular alterations in Leydig cells with germ cell apoptosis in testes of Graomys griseoflavus x Graomys centralis male hybrids. Díaz de Barboza G., Rodríguez V., Ponce R., Theiler G., Maldonado C. and Tolosa de Talamoni, N. Acta Histochemica 116: 604- 614. 2014.

-Past, Present and Future of Melatonin´s Clinical Uses. N. Tolosa de Talamoni, V. Areco, M. Peralta López, G. Díaz de Barboza, M. Rivoira and A. Carpentieri. En: Melatonin and Melatoninergic Drugs in Clinical Practice (Ed. V. Srinivasan et al.). Springer, India. Pág. 27-42. 2014.

Time dependent changes in the intestinal Ca2+ absorption in rats with type I Diabetes mellitus are associated with alterations in the intestinal redox state. Rivoira M., Rodríguez V., Peralta López M. y Tolosa de Talamoni, N. Biochimica et Biophysica Acta. Molecular Basis of Disease 1852:386-94. 2015.

-Molecular aspects of the intestinal calcium absorption. Díaz de Barboza G., Guizzardi S and Tolosa de Talamoni N. World Journal of Gastroenterology 21: 7142- 7154. 2015. (review)

-Dietary and pharmacological compounds altering the intestinal Ca2+ absorption in humans and animals. Areco V., Rivoira M., Rodriguez V., Marchionatti A., Carpentieri A. y Tolosa de Talamoni, N. Nutrition Research Review 28: 83-99. 2015. (review)

-Ursodeoxycholic and deoxycholic acids: differential effects on intestinal Ca2+ uptake, apoptosis and autophagy of rat intestine. Valeria A. Rodríguez, María A. Rivoira, Adriana del V. Pérez, Ana M. Marchionatti and Nori G. Tolosa de Talamoni. Arch. Biochem. and Biophys 591:28-34. 2016.

-Melatonin not only restores but also prevents the inhibition of the intestinal Ca2+ absorption caused by glutathione depleting drugs. Vanessa Areco, Valeria Rodríguez, Ana Marchionatti, Agata Carpentieri y Nori Tolosa de Talamoni. Comp. Biochem. and Physiol. [A] 197: 16-22. 2016.

-Lithocholic acid: a new emergent protector of the intestinal calcium absorption under oxidant conditions. Ana M. Marchionatti, Adriana del V. Pérez, María A. Rivoira, Valeria A. Rodríguez and Nori G. Tolosa de Talamoni. Biochem. and Cell Biol. 95: 273-279. 2017.

-Oxidative stress, antioxidants and intestinal calcium absorption. Díaz de Barboza G., Guizzardi S., Moine L. and Tolosa de Talamoni N. World J. Gastroenterol. 23: 2841-2853. 2017. (review)

-Glutamine protects intestinal calcium absorption against oxidative stress and apoptosis. Moine L., Díaz de Barboza G., Pérez A., Benedetto M. and Tolosa de Talamoni N. Comp. Biochem. and Physiol. [A] 212:64-71. 2017.

-Combined calcitriol and menadione reduces experimental murine triple negative breast tumor. Bohl L., Guizzardi S., Rodriguez V., Hinrichsen L., Rozados V., Cremonezzi D, Tolosa de Talamoni N., Picotto G. Biomedicine and Pharmacotherapy 94: 21-26. 2017.

-Naringin prevents the inhibition of intestinal Ca2+ absorption induced by a fructose rich diet. V. Rodriguez, M. Rivoira, S. Guizzardi and N. Tolosa de Talamoni. Arch. Biochem. and Biophys. 636: 1-10. 2017.

-Naringin prevents bone loss in a rat model of type 1 Diabetes mellitus. M. Rivoira, V. Rodriguez, G. Picotto, R. Battaglino and N. Tolosa de Talamoni. Arch. Biochem. Biophys. 637: 56-63. 2018.

-Molecular mechanisms triggered by bile acids on intestinal Ca2+ absorption. A. Marchionatti, M. Rivoira, V. Rodríguez, A. Pérez and N. Tolosa de Talamoni. Current Medicinal Chemistry 25: 2122-2132. 2018. (review)

-Naringin attenuates liver damage in streptozotocin-induced diabetic rats. Rodriguez V., Plavnik L. y Tolosa de Talamoni, N. Biomed Pharmacother.105:95-102. 2018.

-Glutathione depleting drugs, antioxidants and intestinal calcium absorption. Moine L., Rivoira M., Díaz de Barboza G., Pérez A. and Tolosa de Talamoni, N. World Journal of Gastroenterology. 2018.

-Protective role of melatonin on retinal ganglionar cell: In vitro an in vivo evidences. Del Valle Bessone C, Fajreldines HD, de Barboza GED, Tolosa de Talamoni NG, Allemandi DA, Carpentieri AR, Quinteros DA.  Life Sci. 218:233-240. 2019.

-Glutamine protects both transcellular and paracellular pathways of chick intestinal calcium absorption under oxidant conditions”, L. Moine, A. Pérez, C. Maldonado, N. Tolosa de Talamoni and G. Díaz de Barboza, Comparative Biochem. & Physiol. [A], Mol Integr Physiol.238:110553, 2019.

-Combined treatment of menadione and calcitriol increases the antiproliferative effect by promoting oxidative/nitrosative stress, mitochondrial dysfunction and autophagy in breast cancer MCF-7 cells”, S. Guizzardi, G. Picotto, V. Rodriguez, J. Welsh, C. Narvaez, L. Bohl and N. Tolosa de Talamoni, Canadian Journal of Physiology and Pharmacology, 2020, en prensa.

-Role of mitochondria in the differential action of sodium deoxycholate and ursodeoxycholic acid on rat duodenum», Adriana Pérez, María Angélica Rivoira, Valeria Rodríguez, Ana Marchionatti and Nori Tolosa de Talamoni, Canadian Journal of Physiology and Pharmacology, 2020, en prensa.